https://cirdjournal.com/index.php/pamaj <p>Pharmacology and Alternate Medicine Academic Journal is a multidisciplinary journal based on research and applications of various subject areas related to Pharmacy and Alternative Medicine. PAMAJ publish novel research that is innovative, mechanism-based, hypothesis-driven, and supported by sound experimental design, methodology, and data interpretation.</p> CIRD Publication en-US Pharmacology and Alternate Medicine Academic Journal https://cirdjournal.com/index.php/pamaj/article/view/971 <p><br><strong>Introduction: </strong>Triple-Negative Breast Cancer (TNBC) is clinically aggressive. There is growing evidence that the tumour microenvironment's low pH (acidity) tends to boost cancer aggressiveness. The acidity of the tumour microenvironment tends to correlate with high TNBC chemo-resistance. Strategic manipulation to raise the tumour microenvironment's pH may help discover new anti-cancer therapies. An anhydrous chemical, DFHCOP-A has high pH of 10.5 and is remarkably non-corrosive. However, no known study has established the effect of DFHCOP-A in altering tumour microenvironment pH.<br><strong>Material and Methods:&nbsp;</strong> Ethical clearance was given. Patient-Derived Xenograft (PDX) model was derived from a consenting Nigerian woman with TNBC who had undergone mastectomy. 100mg/kg, 300mg/kg and vehicle of DFHCOP-A were administered to mice models after 1-week xenograft and 6-weeks xenograft in a time/dose-dependent manner.<br><strong>Results and Discussions:</strong> 100mg/kg DFHCOP-A treatment inhibited cancer growth while 300mg/kg DFHCOP-A had no significant effect on cancer growth compared to vehicle control after 1-week xenograft. Paradoxically, there was a significant elevation of serum pH and ionic calcium depletion in the 100mg/kg treated group compared to the vehicle control and 300mg/kg-treated groups.<br><strong>Conclusion:</strong> This study provides preliminary evidence that DFHCOP-A at a low dose may increase the pH of the tumour microenvironment to potentiate cancer drugs.<br>Keywords: Hormesis; Triple Negative Breast Cancer; pH; Tumour microenvironment; patient-derived xenograft; Nigerian woman; DFHCOP-A, ionic calcium, Lactate, Anion Gap, Serum Electrolytes, serum pH</p> Uzoamaka Okoli Michael Okafor Chioli Chijioke Iroka Udeinya Copyright (c) 2022 Pharmacology and Alternate Medicine Academic Journal 2022-10-24 2022-10-24 1 4 1 13